This is a Premium feature of Cowlendar. You need to upgrade your plan if you want to use this feature and you are a free user of Cowlendar.
This is great for when you are running a solo business and are ill/sick running a solo or maybe you simply want to close for the holidays
Steps for blocking a timeslot for all services service
In your Shopify admin dashboard look at the left panel and click on Apps.
Click on Cowlendar.
Click on Settings.
Scroll down a bit and find Block specific dates.
You will add the date you want to block and a start and end time that matches the timeslot you want closed.
Example: If you want to block a timeslot that is on January 10th from 12:00 - 13:30. Then you would add January 10th -> January 10th from 12:00 - 13:30